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KMID : 1041219950370010052
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Journal of Sericultural and Entomological Science
1995 Volume.37 No. 1 p.52 ~ p.56
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Effect of Hemolymph of Silkworm Larvae on the Multiplication of Bombyx mori Nuclear Polyhedrosis Virus in BmN-4 Cells
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Woo Soo-Dong
Kim Woo-Jin
Jin Byung-Rae
Kang Suk-Kwon
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Abstract
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To investigate the effect of hemolymph of silkworm larvae on the multiplication of Bombyx mori nuclear polyhedrosis virus (BmNPV) in BmN-4 cells, BmN-4 cells were infected with BmNPV, which were sequentially Heated with the hemolymph exracted from B. mori larvae. When the culture media TC-100 containing 3% fetal bovine serum was mixed with 10% hemolymph heated at 65$^{\circ}C$ for 30 minutes, the released polyhedra by multiplication of BmNPV in BmN-4 cells were increased more than those of non-treated. However, multiplication of BmNPV in BmN-4 cells treated with non-heated hemolymph was not effective, since non-heated hemolymph was toxic for the cell growth. The result of plaque assay showed that plaque forming units in BmN-4 cells treated with heated hemolymph are significantly increased, suggesting that efficiency of multiplication of BmNPV in BmN-4 cells is due to increase not of cell growth but of infectivity of BmNPV.
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KEYWORD
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Hemolymph, multiplication, BmNPV, BmN-4 cell
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